ANALYTICAL CHEMISTRY

The course is part of the training activities characterizing the Master's Degree Course in Biotechnologies for Sustainable Development and the Environment, aimed at providing students with the appropriate preparation to identify and use the appropriate analytical methodologies for the chemical characterization of the environmental matrices.
The course is divided into theory classes (held in the classroom) and laboratory lessons. The training objectives are:
1. To provide knowledge on advanced coupled analytical techniques for the chemical characterization of the environmental matrices. In particular, for analysis of elements and organic compounds at high or trace and ultra-trace concentrations.
2. To provide knowledge on specific analytical methodologies for the chemical characterization in matter and energy transformation processes
3. To provide knowledge on analytical techniques using sensors and multiparametric probes.
4. To provide in-depth knowledge on the theoretical aspects that underlie the different analytical techniques taken into consideration.
5. Provide knowledge on the instrumental apparatus and develop practical skills in order to perform an experimental measurement with the appropriate accuracy and precision.
6. Develop the ability to propose new approaches in the chemical-analytical field to solve complex problems related to the qualitative and quantitative determination of elements and organic compounds of environmental interest in real matrices.
7. Develop skills to extract analytical information from instrumental signals obtained in matter and energy transformation processes.
8. Develop skills for to extract chemical-analytical information, even with automated and / or "on line" procedures.

The course aims to provide the knowledge and methodological bases necessary for chemical characterization of the environmental matrices in matter and energy transformation processes. The student will acquire a broad spectrum of analytical chemistry knowledge and skills including:

Knowledge and understanding
- Know the fundamental principles and terminologies of advanced analytical techniques in matter and energy transformation processes.
- Know the pre-analytical techniques for the determination of elements and organic compounds at high or trace and ultra-trace concentrations in environmental matrices.
- Know the operating principles of advanced analytical instruments.
- Know the operating principles of sensors and multiparametric probes.

Ability to apply knowledge and understanding
- To understand and correctly use the scientific terminology suitable for advanced analytical methodologies in matter and energy transformation processes. Knowing how to use the fundamental principles in identifying the advanced analytical technique suitable for the qualitative and quantitative determination of elements and organic compounds at high or trace and ultra-trace concentrations in matter and energy transformation processes.
- Knowing how to identify and use suitable pre-analytical procedures suitable for to solve complex problems related to the qualitative and quantitative determination of components in real matrices
- Knowing how to identify and use sensors and multiparametric probes suitable for solving the proposed problems.

Judgment skills
Knowing how to identify and use the advanced analytical chemical methodologies suitable for solving the proposed analytical chemistry problems in matter and energy transformation processes.

Communication skills
Knowing how to communicate both verbally and in writing the obtained results with adequate language.

Learning ability
To know how to use the scientific method as a work tool both for the continuation of the training course and for the professional activity. Knowing how to identify and use advanced analytical methodologies for solving the proposed analytical chemistry problems

Basic knowledge in analitycal chemistry

The course takes into consideration the analytical methodologies for chemical characterization of the environmental matrices (water, sediment, air, soil, biota etc…) in matter and energy transformation processes and the determination of elements and organic compounds at high or trace and ultra-trace concentrations. All the analytical steps are examined from the treatment of the sample to the determination. Will be examined automated and / or "on line" procedures.
Will be examined and used:
- Sampling activities, treatment of abiotic and biotic samples, analysis of elements and organic compounds at high or trace and ultra-trace concentrations.
- Chemical characterization of the environmental matrices (BOD, COD, Organic and Inorganic Carbon, P, N, S compounds)
- Low and high resolution mass spectrometry.
- Gas chromatography coupled with mass spectrometry (high and low resolution).
- Liquid chromatography coupled with mass spectrometry (HPLC-MS / MS, IC / MS, LC-Orbitrap, LC-TOF)
- Inorganic mass spectrometry (ICP-MS).
- Analysis by sensors and multiparametric probes

Presentations provided by the professor.

With the oral examination will be verified :
- Theoretical knowledge of analytical methodologies
- Theoretical knowledge in the use of analytical instrumentation
- Technical knowledge to solve analytical problems

The questions include the description and the solution of analytical problems

The final mark of the teaching activities of course is composed of the arithmetic average of the marks obtained in the two different modules.

Classroom lessons and laboratory activities

Italian

oral

This subject deals with topics related to the macro-area "Climate change and energy" and contributes to the achievement of one or more goals of U. N. Agenda for Sustainable Development