BIOANALYTICAL CHEMISTRY
- Academic year
- 2019/2020 Syllabus of previous years
- Official course title
- CHIMICA BIOANALITICA
- Course code
- CM0386 (AF:305816 AR:166382)
- Modality
- On campus classes
- ECTS credits
- 6
- Degree level
- Master's Degree Programme (DM270)
- Educational sector code
- CHIM/01
- Period
- 2nd Semester
- Course year
- 1
- Where
- VENEZIA
Contribution of the course to the overall degree programme goals
Expected learning outcomes
i) Acquire knowledge on the relationships between molecular structure and methods of analysis of proteins and polynucleotides.
ii) Acquire knowledge of the basic principles of the analytical methods related to enzymatic, immunochemical and nucleic acid hybridization.
iii) Understanding the innovative role that nanotechnologies and nanomaterials are playing in the field of bioanalytical chemistry.
2. Ability to apply knowledge and understanding
i) Understanding which methodologies should be taken into account to optimize the use of biomolecules as analytical reagents to devise a reliable biosensor.
ii) Capability to evaluate which bioanalytical method is the most suitable to solve a specific bioanalytical problem, such as the recognition and the quantitative determination of molecules of biological and medical interest.
3. Ability to judge (depending on the in-depth analysis of the subject matter during the course)
i) Evaluate comparatively the effectiveness of different analytical strategies to choose the most suitable method for qualitative and quantitative analysis of biomolecules or molecules of biological and medical interest.
ii) Develop critical skills in the evaluation of the analytical performances of the methods based on (or devised for) molecules of biological, biotechnological and medical interest.
4. Communication skills
i) Learn the use of the correct scientific terminology of the analytical and bioanalytical field.
ii) Improve the ability to communicate through images by preparing a brief PowerPoint presentation on a topic chosen among those dealt within the course.
iii) Improve the oral communication skills by presenting (10-15 min) and discussing (5-10 min) with the other students and the teacher the above-mentioned PowerPoint presentation.
5. Learning skills
i) Demonstrate to have acquired the principles and concepts on the topics covered by the teacher during the class by implementing the learning process through bibliographic and electronic sources.
Pre-requirements
Contents
- Biological molecules as analytical reagent.
- Enzymatic analysis by spectrophotometry.
- Immobilization of biomolecules: coupling between bioselective layers and analytical transducers.
- Electrochemical and optical biocatalytic sensors. First, second and third generation enzymatic biosensors.
- Immunochemical analysis and immunoassay RIA, EIA, ELISA. Labelled and label-free immunosensors.
- Nucleotides, nucleosides, DNA, RNA. Denaturation, hybridization, intercalation. Analysis of nucleic acid sequence. DNA-Arrays and biochip.
- Micro and nanotechnology in bioanalytics. Use of functionalized nanoparticles for protein analysis and nucleotide sequences
- Applicative examples of commercial bioassays and biosensors.
Referral texts
Lecture notes.
Slides and lecture notes used by the teacher for the lectures will be made available to download from the “Moodle” platform, accessible from the university web-site.
Assessment methods
The oral exam consists of a series of questions, which the student must answer to, thus proving her/his knowldge and understanding of the topics covered by the course.
The correct language and the acquired ability to identify and critically evaluate the appropriateness of the studied bioanalytical techniques will be evaluated.
The oral exam generally lasts approx. 30 minutes, depending on the clarity and appropriateness of the answers to the questions.
Teaching methods
Slides and lecture notes used by the teacher for the lessons will be made available to download from the “Moodle” platform, accessible from the university web-site.
Teaching language
Further information
2. Accessibility, Disability and Inclusion. Accommodation and support services for students with disabilities and students with specific learning impairments:
Ca’ Foscari abides by Italian Law (Law 17/1999; Law 170/2010) regarding supportservices and accommodation available to students with disabilities. This includes students with mobility, visual, hearing and other disabilities (Law 17/1999), and specific learning impairments (Law 170/2010). In the case of disability or impairment that requires accommodations (i.e., alternate testing, readers, note takers or interpreters), please contact the Disability and Accessibility Offices in Student Services: disabilita@unive.it.
Type of exam
2030 Agenda for Sustainable Development Goals
This subject deals with topics related to the macro-area "Human capital, health, education" and contributes to the achievement of one or more goals of U. N. Agenda for Sustainable Development